作者：Xiajing Chen1,2, Yanan Zhang1,2, Hui Zhang1,2, Liang Zhang3, Lingjuan Liu1,2, Yang Cao3, Haitao Ran3 and Jie Tian1,

引用瑞禧生物产品：IMTP (CSTSMLKAC, cyclic form, Mw =943.2 Da) and DSPE

PEG2000-IMTP

 引用瑞禧生物产品：IMTP

Abstract

Background: Ischemic heart disease (IHD) is the leading cause of morbidity and mortality worldwide, and imposes a serious economic load. Thus, it is crucial to perform a timely and accurate diagnosis and monitoring in the early stage of myocardial ischemia. Currently, nanoparticles (NPs) have emerged as promising tools for multimodal imaging, because of their advantages of non-invasion, high-safety, and real-time dynamic imaging, providing valuable information for the diagnosis of heart diseases.

Results: In this study, we prepared a targeted nanoprobe (termed IMTP-Fe3O4-PFH NPs) with enhanced ultrasound (US), photoacoustic (PA), and magnetic resonance (MR) performance for direct and non-invasive visual imaging of ischemic myocardium in a rat model. This successfully designed nanoprobe had excellent properties such as nanoscale size, good stability, phase transformation by acoustic droplet vaporization (ADV), and favorable safety profile. Besides, it realized obvious targeting performance toward hypoxia-injured cells as well as model rat hearts. After injection of NPs through the tail vein of model rats, in vivo imaging results showed a significantly enhanced US/PA/MR signal, well indicating the remarkable feasibility of nanoprobe to distinguish the ischemic myocardium.

Conclusions: IMTP-Fe3O4-PFH NPs may be a promising nanoplatform for early detection of ischemic myocardium and targeted treatment under visualization for the future.

Keywords: Ischemic myocardium, Nanoparticles, Non-invasive, Multimodal imaging

 引用瑞禧生物产品：IMTP

摘要

背景：缺血性心脏病（IHD）是全球发病率和死亡率的主要原因，并带来了沉重的经济负担。因此，在心肌缺血早期进行及时准确的诊断和监测至关重要。目前，纳米颗粒（NPs）已成为多模态成像的有前景的工具，因其具有非侵入性、高安全性和实时动态成像的优点，为心脏病的诊断提供了有价值的信息。

结果：在本研究中，我们制备了一种靶向纳米探针（称为IMTP-Fe3O4-PFH纳米颗粒），其具有增强的超声（US）、光声（PA）和磁共振（MR）性能，可用于直接且无创地可视化大鼠模型中的缺血心肌。这种成功设计的纳米探针具有良好的特性，如纳米级尺寸、良好的稳定性、通过声学液滴蒸发（ADV）实现的相变以及良好的安全性。此外，它对缺氧损伤的细胞以及模型大鼠心脏均表现出明显的靶向性能。通过模型大鼠尾静脉注射纳米颗粒后，体内成像结果显示超声/光声/磁共振信号显著增强，充分表明纳米探针在区分缺血心肌方面具有显著的可行性。

结论：IMTP-Fe3O4-PFH纳米颗粒可能是一种有前景的纳米平台，用于缺血心肌的早期检测以及未来在可视化下的靶向治疗。